金银花水煎液及联合头孢他啶对铜绿假单胞菌生物膜的体外影响

In vitro effects of honeysuckle aqueous-extracts alone and in combination w ith ceftazidime on Pseudomonas aeruginosa biofilm

目的 通过在体外复制铜绿假单胞菌 (Pseudomonasaeruginosa ,P .a)的生物膜 (biofilm ,BF)模型 ,研究金银花水煎液对BF的影响及其与头孢他啶 (ceftazidime ,CAZ)的协同杀菌效果。方法 选取临床分离呼吸道P .a ,采用LB(Luria Bertanimedium)肉汤系统复制体外BF模型 ,扫描电子显微镜(scanningelectronmicroscopy ,SEM)观察BF ,连续稀释法进行活菌计数 ,试管二倍稀释法测定药物的最低抑菌浓度 (MIC)。结果 37℃培养 2 4h ,P .a即表现出对固体表面的黏附性 ,3d形成早期BF ,7d形成成熟BF。金银花组P .a在固体表面的黏附数明显少于空白对照组。 6 2 .5mg/ml的金银花可以抑制和破坏早期及成熟BF ,并增强CAZ对BF内P .a的抗菌活性。结论 金银花水煎液在体外能抑制P .a对固体表面的黏附能力及BF形成能力 ,并能破坏P .a已形成的BF ,增强CAZ对BF内铜绿假单胞菌的清除作用。

Objective To investigate the effect of honeysu ck le aqueous-extracts alone and in combination with ceftazidime(CAZ) on P.aer uginosa biofilm. Methods Clinical isolates of P.aerug inosa were cultured in Luria-Bertani medium for biofilm formation. Biofilm wa s observed in scanning electron microscope(SEM). Viable bacterial counts were de termined by serial dilution. MICs were measured by doubling dilution. Results P.aeruginosa adhered to the surface of the object afte r 24 h incubation and early biofilm was formed in 3 d, mature biofilm was formed in 7 d. The biofilm was inhibited and destructed by 62.5mg/ml of honeysuckle aq ueous-extracts. Conclusion Honeysuckle aqueous-extracts inhibit P.aeruginosa to adhere to the surface of object and inhibit biofilm formation in vitro. It can also destroy the biofilm of P.aeruginosa i n vitro and enhance the bactericidal effect of CAZ on PA within the biofilm. [