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0^#柴油水溶性成分对僧帽牡蛎DNA损伤的初步研究 被引量:17

DNA damage in oyster(Ostrea cucullata) exposed to water-soluble fraction of 0 # diesel fuel
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摘要 在实验条件下,将僧帽牡蛎(Ostreacucullata)分别置于含有低(0.5mg/L)、中(2mg/L)和高(5mg/L)3种浓度0#柴油水溶性成分的海水中,利用碱解旋法测定消化腺和鳃DNA损伤的程度。结果表明,DNA损伤的程度随污染时间的延长而增加,并存在一定的剂量-效应关系;解除污染后,DNA损伤恢复到对照水平。DNA损伤可作为监测海洋石油污染的生物标志物。 To understand the negative effect on ostrea caused by fuel comtamination,an experiment was designed.Ostrea cucullata was exposed to waterˉsoluble fraction of0 # diesel in laboratory condition,and the DNA damage in the digestive gland and gill was measured by alkaline unwinding assay.The results showed:(1)The DNA damage aggravated with prolonged exposure time;(2)DNA damage returned to control level after recovery experiment.DNA damage can be a biomarker to marine oli pollution.
出处 《海洋科学》 CAS CSCD 北大核心 2004年第11期10-14,共5页 Marine Sciences
基金 国家自然科学基金青年基金(20207006) 厦门大学资助项目
关键词 DNA损伤 水溶性成分 牡蛎 初步研究 剂量-效应关系 对照 监测 消化腺 浓度 利用 water-soluble fraction of 0~# diesel fuel Ostrea cucullata DNA damage alkaline unwinding assay
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