草鱼肠道中小肽与血液循环中肽的关系

The relationship between the intestinal small peptides and the peptides amount of blood circulationin Ctenopharyngodon idella

为了研究草鱼肠道完整吸收小肽与肠道中小肽的关系,用6.25%酶解酪蛋白、酸解酪蛋白、酪蛋白溶液和生理盐水进行草鱼肠道灌注试验(1mL·100g-1体重)。20min后尾静脉采血制备血浆,高效液相色谱分析结果表明:灌注酶解酪蛋白溶液的草鱼血液循环中总肽量和某些肽量显著(P<0.05)高于灌注生理盐水、酸解酪蛋白、酪蛋白溶液;较灌注生理盐水草鱼血液循环中总肽量提高18.73%。肠道灌注酸解酪蛋白、酪蛋白溶液对血液循环中总肽量影响不大,无显著性差异(P>0.05)。草鱼血浆中肽量的增加与肠道提供的肽种类和数量有关。实验结果表明,草鱼肠道能够完整地吸收某些小肽进入血液循环。

The study was designed to investigate the special amount of peptides which could be completely absorbed in blood circulation of grass carp. Four groups of grass carp were infused into the front intestine with (6.25%) casein(CA), casein enzymatic hydrolysate(CSP), casein acidized(FAA) solution and normal saline (1mg·100g^(-1) weight). Blood was collected after 20 min infusion from tail vein. The results of analyses by HPLC showed that the total amount of peptides and the amount of some peptides in plasma of grass carp given CSP were significantly higher than those of the grass carp given CA, FAA and normal saline(P<0.05).The total amount of peptides in plasma of grass carp given CSP was increased by (18.73%) compared with those given FAA. There was no significant difference of total amount of peptides in plasma of grass carp given CA, FAA and normal saline(P>0.05).The increase of peptides in plasma was closely related with the kind and amount of peptides in intestine.The study indicated that small peptides could be absorbed into blood circulation of grass carp.