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TPA对Bcl-X前体mRNA在U251细胞中选择性剪接调控的初步研究

Regulation of Pre-mRNA Alternative Splcing in U251 Cells by TPA
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摘要 以含有部分Bcl X序列的微小基因为研究模型 ,研究了TPA对Bcl X前体mRNA选择性剪接在体内和体外的调控作用。通过RT PCR和定点突变实验检测 ,结果表明PKC信号系统能调控Bcl X前体mRNA选择性剪接 ,其机制可能是通过应答序列来调控Bcl X前体mRNA By using the mini-gene construct containing partial sequence of Bcl-X gene as model,we examined the function of TPA on Bcl-X pre-mRNA alternative splicing in vivo and vitro with RT-PCR and site-directed mutagesis assay.The results show that PKA signaling system can regulate Bcl-X pre-mRNA alternative splicing,the possible mechanism is that the responsible sequence affect the choice of the 5′-downstream or upstream splice site of Bcl-X pre-mRNA.
作者 李长友 褚嘉祐 刘晓娟 俞建昆 黄小琴 史荔 朱泳璋 纳剑波 谢久永
机构地区 中国协和医科大学中国医学科学院医学生物学研究所
出处 《Acta Genetica Sinica》 SCIE CAS CSCD 北大核心 2004年第10期1037-1045,共9页
基金 国家"8 63"计划 (编号 :2 0 0 1AA2 2 0 0 41) 国家自然科学基金项目 (编号 :3 9993 42 0 )资助~~
关键词 Bcl-XL/Bcl-Xa 选择性剪接 灯盏花乙素 Bcl-X_L/Bcl-X_s alternative splicing breviscapini
分类号 Q78 [生物学—分子生物学]
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参考文献17

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Acta Genetica Sinica
2004年 第10期

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