荧光定量PCR检测生殖道内沙眼衣原体感染的分析

The Application of PCR in Detection of Chlamydin Trachomatis

目的 :评价DNA定量测定技术在生殖器衣原体感染诊断中的应用价值。方法 :比较DNA定量测定与传统检测手段对衣原体感染的检测结果。结果 :用DNA定量测定法检测衣原体感染 ,其敏感性和特异性均比传统的方法高。定量测定结果显示 ,衣原体感染者最高 2× 10 8copy/ml,最低 2× 10 2 copy/ml。结论 :CTDNA定量测定标本取材要求不是很严格 ,不宜用作短期判愈 。

objective:To evaluate the significance of the fixed quantity of deoxynycleic acid(DNA)in laboratory diagnosis for Chlamydia thachomatic(CT).Methods:The results of the fixed quantity of DNA were compared with those of traditional methods in detecting CT.Results:The sensitivites and specificities of the fixed quantity of deoxynycleic acid were higher than those of traditional culture methods for the testing of CT.The detection of fixed quantity showed patients with CT had 2.0×10 8copy/ml max and 2.0×10 2 copy min.Conclusion:The sampling for the fixed quantity of deoxynycleic is not as strict as that for culture methods.The fixed quantity can not be used for test fure in short time.And it has potential application value for the laboratory diagnosis of CT.