维拉帕米对高糖与去甲肾上腺素诱导的心肌细胞肥大的影响

Effects of Verapamil on Hypertrophic Myocardial Cells Induced with High Glucose and NE

目的 利用体外培养的乳鼠心肌细胞 ,观察维拉帕米对高浓度葡萄糖与去甲肾上腺素共同诱导的肥大心肌细胞的影响 ,并推测其作用机制。方法 以培养的乳鼠心肌细胞为模型分组给药后 ,用 [3 H]leucine标记法测定心肌细胞蛋白的合成 ;利用计算机图象分析系统测心肌细胞的体积。结果 一定浓度的维拉帕米在对高糖和去甲肾上腺素诱导的肥大心肌细胞的蛋白合成及体积均有显著的抑制作用。

Objective To observe the effects of verapamil on the hypertrophic myocardial cells,cultured cardiomyocytes were used to study the inhibitory effects of verapamil on hypertrophy induced with high glucose and NE.Methods Using cultured myocardial cells as a model, The protein synthesis was assayed with leucine intake method; The cardiomyocytes volumes was measured by computer photograph analysis system; the cellular hypertrophy was observed.Results verapamil inhibited cellular protein synthesis and volumes of cultured hypertrophic myocardial cells induced with high glucose and NE.Conclusions 1μmol·L-1 verapamil inhibited cardiac hypertrophy of cultured myocardial cells induced with high glucose and NE. The effective mechanism of verapamil on cardiac hypertrophy need further study.