固定化桔青霉气升式反应器生产核酸酶P_1的研究

Study on Production of Nuclease P_1 by Immobilized Penicilium Citrinum Cell in Three Phase Airlift Reactor

以玉米芯颗粒吸附桔青霉 (Penicilliumcitrinum)孢子 ,再用质量分数为 1.5%的海藻酸钠包埋吸附固定化细胞玉米芯颗粒 ,于摇瓶中进行分批培养 .实验结果表明 :在固定化细胞产酶的条件下 ,培养基中淀粉水解糖浓度为 9g/L ,蛋白胨浓度为 1g/L ,采用气升式反应器 ,产酶发酵周期为 50h ,发酵液中核酸P1 的活力高达 8.4 3mmol·s- 1 ·L- 1 .双载体固定化细胞经 30批次连续重复发酵产酶稳定在较高水平 。

Mycelia of Penicillium citrinum are absorbed by stone of maize and then wrapped up by 1.5% sodium alginate .The nuclease P 1 produced by the immobilized cells are studied in shaking flasks.The experiment shows that the glucose and peptone contents in medium are 9 g/L and 1 g/L respectively. In the three phase airlift reactor, the activity of nuclease P 1 reached 8.43 mmol·s -1 ·L -1 after 50 h culture. In the course of repeated batch culture , the immobilized cells keep high capacity and mechanical strength after 30 batches .