大孔吸附树脂提取纯化杜仲叶绿原酸的研究

Study of extraction and purification of cholorogenic acid from leaves of Eucommia ulmoides with macroporous adsorption resin

目的 提取纯化杜仲叶绿原酸 ,确定最佳吸附树脂吸附 -解吸条件。方法 以乙醇提取液为吸附原液 ,静态吸附实验筛选树脂 ;动态吸附实验确定最佳树脂的吸附 -解吸条件。结果 NKA- 树脂最佳吸附 -解吸条件 :吸附流速 2 m l/ min,原液过柱吸附 2次 ,最佳吸附剂为 4 0 %乙醇。结论 NKA- 树脂是吸附绿原酸的理想吸附剂 ,适宜于产业化生产。

Objective To extract and purify chlorogenic acid from leaves of Eucommia ulmoides and to select the optimal adsorption-elution conditions of resin.Methods Resins and optimal adsorption-elution conditions were selected according to the static adsorption tests and dynamic adsorption tests respectively,with ethanol extract liquid as adsorption crude liquid.Results The optimal adsorption-elution conditions are as follows:adsorption flow rate was 2ml/min,crude liquid flowed through the adsorption column twice,40%ethanol was optimal absorbent.Conclusion NKA-Ⅱ resin was optimal absorbent to extract and purify chlorogenic acid and suitable to be used in industry.