胃癌组织中血管表皮生长因子C表达与血管和淋巴管生成及淋巴转移的关系

A study on the expression of vascular endothelial growth factor C (VEGF-C)tumor angiogenesis lymphangiogenesis and lymphatic metastasis in human gastric cancer

目的探讨胃癌组织中血管表皮生长因子C(VEGF C)表达与血管和淋巴管密度及肿瘤淋巴转移的关系。方法采用免疫组化SP法检测 6 8例胃癌组织中VEGF C、CD 31及淋巴管内皮标记物VEGFR 3,计算VEGF C表达的阳性率及肿瘤微血管和微淋巴管密度。结果淋巴结阳性组中VEGF C阳性率 (70 % )显著高于淋巴结阴性组 (30 % ) ,P <0 0 5 ;与VEGF C阴性组 (2 4 4±2 1 )比较 ,VEGF C阳性组淋巴管密度 (2 9 6± 3 0 )明显增高 ,P <0 0 5 ,而微血管密度在两组之间差异无显著意义 ,P >0 0 5 ;与淋巴结阴性组比较 ,在淋巴结转移阳性组淋巴管密度 (31 6± 2 1 )、微血管密度 (4 0 2± 2 3)均有显著提高 ,P <0 0 5。结论VEGF C主要通过调节胃癌组织微淋巴管的生成而影响胃癌淋巴结转移 ;

Objective To explore the relationship between the expression of VEGF C?micro vessel density(MVD)?lymphatic microvessel density(LMVD) and lymphatic metastasis in gastric cancer. Methods Immunohistochemistry (SP) was used to detect the expression of VEGF C?CD 31 and lymphatic endothelium marker VEGFR3 in 68 samples of gastric cancer. ResultsThe positive rates of VEGF C in positive lymph node increased significantly than in negative lymph node (70% vs. 30%, P <0 05); LMVD in positive VEGF C was significantly higher than in negative VEGF C (29 6±3 0)vs. (24 4±2 1), P <0 05. Compared with that without lymph node metastasis, LMVD(31 6±2 1)?MVD (40 2±2 3) in positive lymph node increased significantly, P <0 05).Conclusions VEGF C mediates lymphatic metastasis of human gastric cancer by way of lymphangiogenesis. MVD and LMVD were significant predictive factors for tumor lymphatic metastasis.