表皮生长因子对Balb/3T3成纤维细胞生长周期及表皮生长因子受体表达的影响

EFFECTS OF EPIDERMAL GROWTH FACTOR ON BALB/3T3 FIBROBLAST CELL-CYCLE AND EXPRESSION OF EPIDERMAL GROWTH FACTOR RECEPTOR

目的 :观察表皮生长因子 (Epidermalgrowthfactor ,EGF)对Balb/ 3T3成纤维细胞生长周期及表皮生长因子受体(Epidermalgrowthfactorreceptor,EGFR)表达的影响。方法 :①通过血清饥饿法使细胞同步化 ,加入 2 5ng/ml的EGF予以刺激 ,用流式细胞仪检测不同时间细胞生长周期参数的改变 ;②于不同时间点对Balb/ 3T3细胞进行EGFR免疫组化染色 ,观察EGF对EGFR表达的调节作用。结果 :①Balb/ 3T3细胞在加入EGF 8h后S期细胞比例及增殖指数达到最高峰 ,至 1 0h回落至原水平 ;②EGF在作用早期可促进成纤维细胞EGFR表达 ,其对受体表达的调节作用与正常培养液相当。结论 :EGF可有效促进EGFR的表达 。

Objective:.To observe the effect of epidermal growth factor(EGF) on Balb/3T3 fibroblast cell-cycle and the expression of EGFR.Methods:25 ng/ml EGF was added to Balb/3T3 cell in G 0 phase and then the cell-cycle parameters were detected utilizing flow cytometry at various time points.The effects of EGF on the expression of EGFR in Balb/3T3 cell at various time points were observed by means of immunohistochemical staining.Results:After 8 h of EGF stimulation,the proportion of S phase and the proliferation index of Balb/3T3 fibroblast increased to the maximum,then returned to the original level at 10 h.EGF prominently promoted the expression of EGFR in Balb/3T3 fibroblasts in the incipient time and the effect of EGF stimulation was equivalent to that of culture media.Conclusion:The results indicate that EGF can effectively stimulate Balb/3T3 fibroblast proliferation by increasing the EGFR expression.