快速眼动睡眠剥夺后大鼠皮质及海马HSP70表达的研究

<title>pid eye movement sleep deprivation on expression of heat shock protein 70 in rat cortex and hippocampus

目的探讨不同时间的快速眼动(REM)睡眠剥夺对大鼠皮质及海马各区的热休克蛋白70(HSP70)表达的影响及意义。方法60只Wistar大鼠,随机分为睡眠剥夺组(SD)、环境对照组(TC)和空白对照组(CC)。其中SD组又分为1 d、3 d、5 d、7 d 4个时点。用改良多平台睡眠剥夺法进行REM睡眠剥夺,运用免疫组织化学方法观察REM睡眠剥夺后大鼠额叶、顶叶皮质及海马各区HSP70表达的分布规律及时空变化;同时结合蛋白质免疫印记(Western Blot)实验对额叶皮质及全海马HSP70蛋白作了选择性的半定量分析。结果REM睡眠剥夺后1 d脑内HSP70表达一过性增强,以后逐渐下降。Western Blot实验印证了这一结果。结论REM睡眠剥夺能够引起大鼠皮质及海马神经元内HSP70表达增强,可能是一种自身稳定调节的保护机制。

<abstract>jective To observe the spacial and temporal changes of heat shock protein 70 (HSP70) expression in the rat cerebral cortex and hippocampal regions on different stages of rapid eye movement sleep deprivation. Methods The modified multiple platform methods (MMPM) were established as the rapid eye movement ( REM) sleep deprivation method. Totally 60 wister rats were involved and divided randomly into three groups, as cage control (CC) , tank control (TC) and the sleep derivation group (SD). The SD group animals were sacrificed at the end of1d,3d,5d,7d sleep deprivation periods respectively. The ABC immunohistochemistry was used in detecting HSP70 expression and distribution in the rats frontal and parietal cortex, and the different regions of hippocampal. Meanwhile, Western blot technique was specially used to the semi-quantity protein level in frontal cortex and the whole hippocampus. Results The expression of HSP70 in cerebral cortex and hippocampus was increased strongly after 1 d REM sleep deprivation. The Western blot results showed the same trend in frontal cortex. Conclusions REM sleep deprivation might transiently increase the expression of HSP70 in the rat brain. And the widespread increase of heat shock protein family expression might be a neuroprotective response to the prolonged wakefulness.