熟地黄对毁损下丘脑弓状核大鼠学习记忆及下丘脑-垂体肾上腺-海马轴的影响

Effect of Radix Rehmanniae Preparata on the Expression of c-fos and NGF in Hippocampi and Learning and Memory in Rats with Damaged Thalamic Arcuate Nucleus

目的 :观察滋阴药熟地黄益智作用及机理。方法 :采用谷氨酸单钠 (MSG)毁损下丘脑弓状核模型作为肾阴虚学习记忆障碍模型 ;跳台法和Morris水迷宫法观察熟地黄对学习记忆的影响 ;放免法测定血浆ACTH和CORT含量 ,原位杂交观察海马糖皮质激素受体mRNA(GRmRNA)变化 ;测量体温。结果 :熟地黄明显改善MSG大鼠被动回避和空间记忆能力 ,抑制血浆CORT含量和海马GRmRNA表达 ,抑制基础体温升高。结论 :熟地黄具有改善学习记忆作用 ,其机理与抑制血浆CORT含量和海马GRmRNA表达有关。

Objective:To study the effect of Radix Rehmanniae Preparata (RRP) on improving the function of learning and memory.Method:With injured thalamic arcuate nucleus rats induced by MSG,the improving function of RRP on learning and memory was observed by step down task and Morris water maze task,and the expression of c-fos and NGF in hippocampi was observed by immunohistochemical means.Result:RRP could decrease the times of mistakes and prolong the incubation period in step down task,shorten the incubation period of seeking the platform,and improve the rate through the platform position in Morris water maze task.RRP could also increase the expression of hippocampal NGF,c-fos.Conclusion:RRP could improve the function of learning and memory of MSG rats,and its mechanism may by related with the increase of the expression of hippocampal c-fos and NGF.