发酵黑麦糠对右旋葡聚糖硫酸钠诱导的小鼠结肠炎的保护作用

Protective Effects of Fermented Rye Bran against Mice Colitis Induced by Dextran Sulfate Sodium

背景:溃疡性结肠炎是一种病因和发病机制尚不明确的结肠黏膜和黏膜下层慢性炎症。体内外试实验表明,发酵黑麦糠可抑制幽门螺杆菌(H.pylori)对胃黏膜的黏附作用,从而保护小鼠免受H.pylori感染。目的:明确发酵黑麦糠是否对右旋葡聚糖硫酸钠(DSS)诱导的小鼠结肠炎有保护作用。方法:以3.5%DSS诱导小鼠产生结肠炎,部分小鼠同时加用发酵黑麦糠,观察结肠炎小鼠的临床表现(体重下降情况、直肠出血情况和有无腹泻)和结肠黏膜的组织学改变。结果:DSS+发酵黑麦糠组小鼠结肠炎起病晚、症状轻,在观察期内无一例出现腹泻;组织学检查和评分结果亦显示该组小鼠结肠黏膜炎症较DSS组轻。结论:发酵黑麦糠对DSS诱导的小鼠结肠炎有保护作用,其具体作用机制尚有待进一步明确。

The pathology of ulcerative colitis shows the chronic inflammation of colonic mucosa and submucosa, its precise etio-pathogensis remains unclarified. Fermented rye bran has been shown in vitro and in vivo to inhibit the adhesion of Helicobacter pylori (H. pylori) to gastric mucosa, and protect the mice against H. pylori infection. Aims: To investigate whether the fermented rye bran can protect the model mice receiving dextran sulfate sodium (DSS) from colitis. Methods: Colonic inflammation in the mice was induced by exposure to 3.5% DSS. Some mice received a combination of DSS and fermented rye bran. The clinical features including weight loss, rectal bleeding and diarrhea, and the histological changes of colonic mucosa were obseved. Results: The mice exposed to DSS and fermented rye bran showed late onset of the illnese and milder clinical symptoms, no diarrhea occurred during the period of observation. Histologically, the colonic mucosal inflammation in the mice receiving DSS and fermented rye bran was much less severe than that in the mice receiving DSS only. Conclusions: Fermented rye bran can somehow protect against the development of DSS-induced colitis in mice. Further studies are needed to clarify the mechanisms of its action.