摘要
目的 观察携带人非分泌型尿激酶型纤溶酶原激活物cDNA的复制缺陷型腺病毒pAd-△uPA对大鼠肝纤维化的影响。方法 运用DNA重组技术,构建携带人非分泌型尿激酶型纤溶酶原激活物cDNA的复制缺陷型腺病毒pAd-△uPA,通过门静脉注射将其导入大鼠肝纤维化模型体内;通过逆转录一聚合酶链反应(RT-PCR),Ⅰ、Ⅲ型胶原的免疫组织化学以及Von Gieson胶原染色法观察腺病毒pAd-△uPA对大鼠肝纤维化的影响。结果pAd-△uPA可在大鼠肝脏中表达,其表达产物可促进肝脏中Ⅰ、Ⅲ型胶原的降解(P<0.05),但对病理形态学的影响尚不显著(P>0.05)。结论腺病毒pAd-△uPA可促进肝纤维化肝脏中Ⅰ、Ⅲ型胶原的降解。
Objective To observe the effects of recombinant adenoviruses carrying human urokinase-type plasminogen activator on experimental liver fibrosis. Methods We constructed replication-deficient recombinant adenoviruse (pAd-△uPA) inserted with non-secreted human urokinase-plasminogen activator cDNA which can be expressed in eukaryotic cells, pAd-△uPA was transferred to the rat's fibrotic liver in vivo by portal vein, then the effects of the pAd-△uPA on the fibrotic liver were observed by RTPCR, immunohistochemistry and pathology. Results The pAd-△uPA could be expressed in rat liver. The expression of the pAd-△uPA could increase the degradation of type Ⅰ and type Ⅲ collagen in the rat's fibrotic liver as compared with fibrotic model group (P<0.05). However according to the pathological observation, the pAd-△uPA had little reverse effects on the rat's fibrotic liver as compared with fibrotic model group (P>0.05). Conclusion The pAd-△uPA have some effects on liver fibrosis, but the effects are limited.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2004年第8期942-944,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金(30271270)
