川芎嗪对离体兔阴茎海绵体平滑肌条收缩的影响

Effects of ligustrazine on the contraction of isolated rabbit corpus caverno sum strips

目的 初步探讨川芎嗪(Ligustrazine)对离体兔阴茎海绵体平滑肌条的舒张作用及其机制。方法 离体家兔阴茎海绵体平滑肌实验方法,观察川芎嗪对阴茎海绵体平滑肌的舒张效应,测定去氧肾上腺素(PE)和氯化钾(Ka)的浓度-效应曲线。结果 川芎嗪浓度依赖性地舒张PE诱发的阴茎海绵体平滑肌收缩作用,最大舒张效应为(74.1±6.2)％[对照组为(21.9±5.6)％,P<0.01]。不同浓度的川芎嗪可使PE和KCL的浓度-效应曲线右移,最大收缩反应降低,高浓度(0.8g/L)时抑制收缩作用更显著。结论 川芎嗪有明显的舒张阴茎海绵体平滑肌作用,并呈剂量依赖性。川芎嗪舒张阴茎海绵体平滑肌机制可能与抑制细胞外钙内流有关。

Objective To investigate the role and mechanism of ligustrazine on relaxing the isolated rabbit corpus cavernosum tissue. Methods The relaxant effects of ligustrazine on corpus cavernosum were observed by using experimental method of smooth muscle strips. Concentration-responses to PE and KC1 were recorded. Results Ligustrazine concentration-dependently depressed the contraction response to PE. The maximum percentage relaxation of cavernosal strips by ligustrazine was (74.1±6.2)% [ compared with control: (21.9±5.6)%, P<0.01 ]. Ligustrazine concentration-dependently inhibits the amplitude of the contraction induced by cumulative doses of PE and KC1. Conclusion Ligustrazine significantly relaxes the cavernosal muscle contraction induced by PE in vitro. The results suggested that ligustrazine inhibit calcium ion influx.