人脑胶质瘤血管生成素-2表达及其与血管生成和脑水肿的关系

The expression of angiopoietin-2 in human brain glioma and its relationship with angiogenesis and brain edema

目的 研究血管生成素.2(Ang-2)基因在人脑胶质瘤表达及其与胶质瘤血管生成及瘤周水肿的关系。方法 用半定量逆转录-聚合酶链反应(RT-PCR)、免疫组织化学方法测定42例人脑胶质瘤和8例正常脑组织中Ang-2 mRNA及其蛋白表达情况。用免疫组织化学方法检测肿瘤微血管密度(MVD)。结果 正常脑组织中无或弱表达Ang-2。42例胶质瘤组织中均有Ang-2 mRNA表达,不同级别间Ang-2 mRNA的表达差异有显著性(P<0.05)。随着脑胶质瘤恶性程度的增加,Ang-2 mRNA的表达增高(r=0.894,P<0.01)。免疫组织化学结果显示,胶质瘤细胞及肿瘤血管内皮细胞中均有Ang-2蛋白表达。Ang-2 mRNA表达与MVD、脑水肿指数(EI)显著相关(分别为r=0.853,P<0.01;r=0.784,P<0.01)。结论 Ang-2可能参与胶质瘤血管生成,对胶质瘤瘤周脑水肿及恶性进展有促进作用。

Objective To investigate the expression of Ang-2 gene in glioma and its relationship with angiogenesis and brain edema. Methods The expression of Ang-2 in 42 human gliomas and 8 normal brain tissues were detected by RT-PCR and immunohistoehemical method. Intratumoral microvessel density (MVD) was examined by immunohistochemical staining. Results There was weak or no Ang-2 expression in normal brain tissues. The expression of Ang-2 mRNA was detectable in all of 42 gliomas. There was significant difference in the expression of Ang-2 mRNA among different grades (P<0.05). The expression level of VEGF mRNA was increased with the increase of tumor pathological grade ( r =0.894, P<0.01 ). Immunohistochemical staining revealed the existence of Ang-2 protein in both tumor cells and endothelial cells of glioma. Ang-2 mRNA expression was significantly positively correlated with MVD and edema index (EI) ( r = 0.853, P < 0.01 ; r = 0.784, P < 0.01, respectively). Conclusion Ang-2 might play an important role in the peritumoral brain edema and the progression of glioma by pro- moting angiogenesis.