兔下颌牵张成骨术中激活素A和卵泡抑素mRNA表达的研究

mRNA EXPRESSION OF ACTIVIN A AND FOLLISTATIN IN RABBIT MANDIBULAR DISTRACTION OSTEOGENESIS

目的 在兔下颌骨牵张模型中检测不同时期激活素 A( activin,ACT A)和卵泡抑素 ( follistatin,FS)m RNA的表达 ,探讨 ACT A/ FS系统对下颌牵张成骨的调节模式。 方法 选用雌性健康大白兔 2 8只 ,制作兔右侧下颌骨牵张模型 ;术后 7天开始牵张 ,牵张速度为每天 1mm ,每只动物右侧下颌骨延长 10 mm。在延迟期末及牵张期末固定 10、2 0、30、4 0和 6 0天 ,切取下颌骨的新生骨组织及对侧正常下颌骨组织提取 RNA,用 RT- PCR检测 ACT A、FSm RNA各时期的表达。 结果 正常下颌骨组织未检测出 ACT A m RNA,随着牵张过程开始 ACT A m RNA的表达量逐渐上升 ,固定 10天和 2 0天达峰值 ,分别为延迟期末的 5 .0 4和 4 .98倍。下颌骨牵张成骨过程中 FSm RNA表达的总趋势与 ACT A m RNA基本相同。 结论 在兔下颌骨牵张过程中 ACT A/

Objective To examine the mRNA expression of activin A(ACT A) and follistatin(FS) during mandibular lengthening and to elucidate the regulating pattern of during mandibular distraction osteogenesis.Methods Skeletally mature white New Zealand rabbits were established right mandibular distraction osteogenesis models and the mandibles were lengthened 7 days after osteomy. At the end of latency period and the end of distraction period, 10,20, 30, 40 and 60 days after fixation, the regenerating tissue of animals' lengthened mandibles and that of the other side normal mandibles were harvested to extract RNA and to analyse ACT A, FS mRNA by RT PCR.Results The expression of ACT A mRNA was not detectable in normal bone tissue and ACT A mRNA began to express at the end of latency period. The expression of ACT A mRNA increased gradually along with the beginning of distraction and reached the peak on the 10th and 20th days of distraction which was 5.04 and 4.98 times as much as that of the end of latency period, respectively. The trend of expression of FS mRNA during mandibular distraction osteogenesis was the same as expression of ACT A mRNA. Conclusion ACT A/FS play an important role during rabbit mandibular distraction osteogenesis.