生物衍生羊膜对大鼠跟腱粘连影响的实验研究

EFFECT OF HUMAN ACELLULAR AMNION MEMBRANE ON TENDON ADHESION IN RAT

目的 研究生物衍生羊膜预防 SD大鼠跟腱粘连的效果 ,并为临床预防术后跟腱粘连提供实验数据。方法 以 2 8只 SD大鼠后肢跟腱为实验模型 ,切断并缝合跟腱 ,实验侧跟腱包裹生物衍生羊膜 ,对侧不包裹作为对照 ,术后 1、2、4、6、8和 12周分别取跟腱 ,大体及组织学观察跟腱的粘连、愈合情况。 结果 术后各时间点两侧跟腱吻合处炎性细胞浸润情况、跟腱愈合进程无明显差别 ,跟腱粘连的半定量评分表明实验侧和对照侧差异有统计学意义 ( P<0 .0 5 )。 结论 生物衍生羊膜能有效预防跟腱粘连 ,是一种较为理想的预防跟腱粘连的生物材料。

Objective To investigate the effects of human acellular amnion membrane on SD rat tendon adhesion and to obtain the experimental data for clinical application in preventing postoperative tendon adhesion. Methods The tendons of 28 adult SD rats hindlimb were cut and sutured. The tendons of left hindlimb were encapsulated by human accellular amnion membrane as the experimental group and the ones of the other side were not encapsulated as control group. The rats were killed 1, 2, 4, 6, 8 and 12 weeks after operation. The results were evaluated grossly and histologically. Results There were no differences in healing of injury tendon and inflammatory response between the two groups. The anatomical and histological results showed the experimental group had less adhesion than the control group( P <0.05). Conclusion Human acellular amnion membrane can prevent adhesion of tendon without affecting tendon healing and is an optimal biological material to prevent tendon adhesion.