白蛋白对内皮细胞增殖的影响

Effect of albumin on proliferation of EC304

目的 :观察白蛋白 (albumin ,ALB)对内皮细胞增殖的影响。方法 :加入不同终浓度的BSA和HSA(2 0 %FCSRPMI- 16 4 0 )培养液 ,37℃、5 %CO2 、10 0 %饱和湿度培养一定时间后 ,采用MTT法于酶联免疫测定仪4 90nm波长处测定A值 ,评价内皮细胞株 (EC30 4 )的生长状态。结果 :不同浓度的BSA和HSA对 (ECV30 4 )的作用相近 ,均能促进内皮细胞生长 (P <0 0 1)。结论 :白蛋白对EC生长有明显促进作用。

Objective:To observe the influence of albumin (ALB) on EC proflieration.Method:EC304 was incubated in 20% FCS RPMI-1640 culture medium containing different concentration of BSA and HSA at 37℃,5%CO 2 for 24 or 48 hours.The proliferation of EC was analyzed by MTT method (490nm).Result:The promoting proliferaiton of EC304 was observed in media containing BSA or HSA in different concentration (P<0.01).conclusions:Albumin could accelerate the proliferation of EC.