摘要
目的 :探讨 BL U基因在 T/ NK细胞淋巴瘤发生中的作用与分子病理诊断中的应用价值。方法 :选用已经确诊为鼻 T/ NK细胞淋巴瘤 1 2例的冻存组织标本与 2 0例石蜡组织标本 ,从组织中提取 DNA,用 MSP方法检测 BL U基因启动子的甲基化状态。结果 :在 1 2个冰冻组织病例中 ,用 M引物扩增的产物 ,BLU基因全部为阳性 ,U引物扩增的产物 ,BL U基因 1 1例为阳性 ;2 0例石蜡组织病例中 ,M引物扩增的产物 6例阳性 ,U引物扩增的产物 1 2例阳性。表明 BLU基因在鼻 T/ NK细胞淋巴瘤中有高度甲基化 ,冰冻组织中的甲基化检出率明显高于石蜡组织。结论 :BL U基因启动子 Cp G岛在鼻 T/ NK细胞淋巴瘤中普遍高度甲基化 ,表明此抑癌基因被失活 ,可能是 T/ NK细胞淋巴瘤的发病机制之一。
Objective:BLU are candidates tumor suppressor gene(TSG) involved in the negative control cell cycle by means of alternative transcripts. We intend to investigate the hypermethylation of the BLU promoter in lymphoma from nasopharyngeal mucosa to explore its value in tumorigenesis and molecular pathology diagnosis. Methods: All cases were studied by using methylation specific PCR(MSP).DNA was extracted from 12 frozen tissues and 20 paraffine-embedded tissues of nasal T/NK cell lymphomas,then treated by sodium bisulfite and PCR amplified by specific primers for methylated versus unmethylated DNA. PCR productions were visualized under UV after agarose electrophresis. Results: The primers were designed to discriminate between methylated and unmethylated alleles following bisufite treatment and to discriminate between DNA modified by bisulfite and DNA which had not been modified. Hypermethylation of BLU gene promoter is detected in 12 out of 12 frozen tissue cases (100%) and in 6 out of 20 paraffine-embedded tissue cases(30%) of nasal T/NK cell lymphoma. The ratio of detection from frozen tissue is higher than from paraffin-embedded tissue. Conclusion:In this study ,we demonstrate the use of MSP to identify BLU gene promoter hypermethylation in nasal T/NK cell lymphoma. The result show that the BLU hypermethylation may be one of the pathogenetic mechanisms. Analysis of BLU gene methylation in tumors may help us to improve the molecular diagnosis of this lymphoma. Frozen tissue should be better than paraffin embedded tissue.
出处
《陕西医学杂志》
CAS
北大核心
2004年第9期771-774,共4页
Shaanxi Medical Journal
