摘要
在GM玻璃纸平板上接种10~6数量级的孢子悬液,31℃培养17～18hr,收集菌丝于1.5%Lywallzyme中酶解4hr,过滤离心收集原生质体,原生质体经50℃,5min处理,并20W紫外灯40cm照射,150s,0.1MHNO_2,pH4.6.再经31℃恒温处理7min,原生质体可达到全部灭活.
Spore liguor(10°per ml)vaccinated on cellophane planch GM.cultivated
at 31℃ for 17～18 hr.Mycelia were gathered and enzymed with 1.5%(g/ml)Lywallzyme for 4 hr.Protoplasts,which were obtained by the process of filtering and centrifugating,were blanched when dealt with heat at 50℃ for 5 min of 20W ultraviolet-ray 40cm for 150 sec-onds,or 0.1 M HNO_2,pH4.6,31℃ for 7 min.
出处
《湖南师范大学自然科学学报》
CAS
1993年第1期68-71,共4页
Journal of Natural Science of Hunan Normal University
关键词
黑曲霉
原生质体
灭活
真菌
Aspergillus niger
protoplast
blanching
