抗胃癌抗体轻链可变区序列测定和三维结构模建

Sequencing of light chain variable region antibody against human gastric cancer and modeling of its three dimensional structure

目的:确定抗胃癌抗体轻链可变区(VL)的一级结构,并模建其三级结构. 方法:从抗人胃癌噬菌体抗体库中筛选出VL基因,并进行序列测定和分析.利用同源蛋白结构模建技术,采用计算机辅助分子设计系统模建VL的三维空间结构. 结果:所获得的VL序列符合鼠抗体可变区特征,并成功构建了其三维空间结构. 结论:所测得的VL一级结构和构建的三维空间结构均有较高的可靠性.

AIM: To confirm the primary structure and to model the three-dimensional structure of the light chain variable region (VL) antibody against human gastric cancer. METHODS: The VL gene was selected from the phage display library of antibody against human gastric cancer, se-quenced and analyzed. The three dimensional structure of VL was also modeled by computer homology modeling techniques. RESULTS: The sequence of VL was in agreement with the characteristics of the mouse antibody variable region. In addition, the three dimensional structure of VL was modeled. CONCLUSlON:The primary structure and the three-dimensional structure of VL are proved reasonable and reliable.