散发性大肠腺癌易感性与吸烟和GSTT1空白基因型

Correlation of GSTT1 gene polymorphism and smoking with susceptibility to sporadic colorectal adenocarcinoma in China

目的:探讨谷胱甘肽S-转移酶(GST)T1空白基因型和吸烟与散发性大肠腺癌(SCRAC)遗传易感性关联. 方法:应用多重PCR技术,检测GSTT1基因多态性. 结果:GSTT1空白基因型在吸烟大于10支/d的对照组和SCRAC组中的频率差异有显著性(X2=5.35,P=0.021), 在重度吸烟的远端SCRAC(X2=6.48,P=0.011)、非老年人SCRAC(X2=4.53,P=0.033)及低分化肿瘤(X2=4.02, P=0.045)与重度吸烟的对照组之间的频率差异均有显著性,不同Dukes分期重度吸烟的SCRAC分别与重度吸烟的对照组比较,GSTT1空白基因型频率的差异无显著性. 结论:GSTT1空白基因型提高了重度吸烟的个体患SCRAC 的危险陛,GSTT1空白基因型与重度吸烟的远端SCRAC 的易感性之间存在关联,肿瘤多见于非老年患者,多呈低分化性腺癌.

AIM: To analyze the association genetic polymorphisms of glutathione S-transferase T1 (GSTT1) and smoking with genetic susceptibility to sporadic colorectal adenocarci-noma (SCRAC). METHODS: All subjects were unrelated Han people in Hubei province of China. Using multiplex PCR, we studied the genetic polymorphisms of the GSTT1 genes. RESULTS: The difference of frequency of GSTT1 null genotype between severely-smoking SCRAC and the severely-smoking controls reached statistical significance (X2=5.35, P =0.021). The frequency of GSTT1 null genotype was more common in the distal SCRAC with severely-smoking (X2=6.48, P =0.011), in the non-elder SCRAC with severely-smoking (X2=4.53, P =0.033), and in the poorly-differentiated SCRAC with severely-smoking (X2=4.02, P =0.045) when compared with the severely-smoking controls. On the other hand, compared with the severely-smoking controls, all the difference of frequency of GSTT1 null genotype in severely-smoking SCRAC with various Dukes stages did not reach statistical significance, respectively. CONCLUSION: The GSTT1 null genotype may augment the risk of SCRAC with severely-smoking, especially the non-elder patients, and most tumors are distal SCRACs and poorly-differentiated SCRAC.