c-fos、c-jun蛋白在口腔黏膜下纤维性变组织中的表达研究

Study on the expression of c-fos and c-jun protein in oral submucous fibrosis

目的 :检测c -fos、c -jun蛋白在口腔黏膜下纤维性变 (OSF)组织中的表达及分布 ,探讨其在OSF发病机制中的作用。方法 :采用免疫组化SABC法 ,用c -fos、c -jun兔抗人多克隆抗体检测 5 6例OSF病变组织、15例口腔黏膜扁平苔藓 (OLP)病变组织及 10例正常口腔黏膜组织中的c -fos、c -jun蛋白的表达及分布。 结果 :c -fos、c -jun蛋白在OSF病变组织有明显的阳性表达 ,正常口腔黏膜及OLP组织为阴性表达 (P <0 .0 5 )。其表达主要位于OSF病变上皮组织 (P <0 .0 5 ) ,且表达强度为正相关 (γs=0 .744 ,P <0 .0 1)。结论 :c -fos、c -jun蛋白在OSF病变组织中高表达 ,在OSF发病机制中有重要作用。

Objective:this study aim to evaluate expression of c-fos and c-jun protein in Oral Submucous Fibrosis ,and investigate their possible role in Oral Submucous Fibrosis.Method: Expression of protein of c-fos and c-jun in 56 cases of Oral Submucous Fibrosis (OSF),15 cases of oral lichen planus (OLP) and 10 cases of normal contral oral mucosa-tissue were studied by SABC immunohistochemistry with the c-fos、c-jun rabbit anti human polyclonal antibody.Result:The study showed that the c-fos and c-jun-immunoreactivity were seen significantly in OSF compared to normal contral and OLP group which were negative expression(p<0.05). they were found mainly in epithelial cells(P>05), There was positive correlation between the expression of c-fos and c-jun protein(γs=0.744,P<0.01).Conclusion: c-fos and c-jun protein have positive expression in Oral Submucous Fibrosis , they act as an important role in pathogenesis of Oral Submucous Fibrosis.