MSCs移植促进输送盘骨牵张术修复颅骨缺损的实验研究

Effects of transplantation of autologous MSCs on transport osteodistraction in a goat calvarial defect model

目的 :研究自体骨髓间充质干细胞 (MSCs)对采用输送盘骨牵张术修复山羊颅骨缺损效果的影响。方法 :将成年雄性山羊 8只 ,随机分为A、B两组 ,每组 4只。在动物颅顶区制造全层颅骨缺损和输送盘 ,用自行研制的牵张器行输送盘骨牵张术修复颅骨缺损。牵张结束后 ,在A组动物牵张区注入体外培养的自体骨髓间充质干细胞 ;B组同期注入生理盐水作对照。在牵张结束后的第 4周处死A、B两组动物 ,取牵张区新生组织标本行组织学和扫描电镜分析。结果 :移植MSCs的A组动物牵张区新骨生成速度和钙化程度显著高于B组动物。结论 :局部移植MSCs可能有促进牵张成骨的作用。

Objective:To study the effects of mesenchymal stem cells(MSCs) transplantation on new bone formation during transport distraction osteogenesis in a goat model of calvarial defect.Method:8 adult goats were randomly divided into group A (n=4) and group B(n=4) .A calvarial defect and a transport disk were created in all animals , and a custom-made distractor was used for repairing the cranial defect. The group A goats were injected with autologous MSCs into the distracted region, whereas the group B with injection of physiological saline were used as controls. At 4 weeks after end of distraction, all animals were killed, and the distracted callus was obtained and prepared for histological and scanning electron microscopy analysis.Result:The new bone formation in the distraction gap at group A was superior to that of group B after transport osteodistraction for repairing of calvarial defect.Conclusion: Transplantation of autologous MSCs may have beneficial effect on bone formation following transport distraction osteogenesis in a goat model of calvarail defect.