稳定表达cbfa1的人牙乳头细胞模型的建立和鉴定

Establishment and identification of human dental papilla cells model in which cbfa1 expressed stably

目的 :建立能稳定表达核心结合因子a1(cbfa1)的人牙乳头细胞模型。方法 :体外培养人牙乳头细胞 ,脂质体法转染重组质粒 ,G418筛选抗性克隆 ,并从基因 (原位杂交和RT -PCR)和蛋白水平 (免疫组化和westernblot)进行鉴定。结果 :共获得三个G418抗性克隆 ,其中PC -3克隆能够稳定表达cbfa1基因和蛋白。结论 :成功建立了稳定表达核心结合因子a1的人牙乳头细胞模型PC -3。

Objective:To establish human dental papilla cells model, in which cbfa1 gene and proteins expressed stably.Method:we transfected cbfa1 recombinant plasmids into cultured dental papilla cells by lipofectamin in vitro and filtrated by G418. Then RT-PCR, in situ hybridization, immuneohistochemistry and western blot were performed to identify the cells model.Result:In three G418 fastness cells models, only one can stably express cbfa1 mRNA and proteins.Conclusion: We established human dental papilla cells model named PC-3 successfully.