旋毛虫新生幼虫cDNA文库的免疫筛选

Immunoscreening of Newborn Larvae cDNA Library of Trichinella spiralis

目的 获取具有抗原性的旋毛虫新生幼虫基因。 方法 以旋毛虫感染猪血清为抗体探针 ,对旋毛虫新生幼虫cDNA文库进行免疫筛选 ,将获得的阳性克隆进行测序及分析。 结果 从 4 5× 10 5旋毛虫新生幼虫cDNA文库重组子中共获得 15 8个阳性克隆。序列分析结果表明 ,其中有 3 6个新的cDNA序列 ,已报道的序列有 5个 ,有 12个序列无开放阅读框架 (ORF) ,与旋毛虫线粒体基因组DNA同源。

Objective To obtain antigenic genes encoding newborn larvae antigens of Trichinella spiralis. \ Methods\ Newborn larvae(NBL) cDNA library of Trichinella spiralis was screened using the infected swine serum and positive clones were sequenced and analysed. \ Results \ 158 positive clones were obtained from 4\^5×10\+5 recombinant clones by immunoscreening. The sequence analysis of positive clones showed that there are 36 novel cDNAs, 5 reported cDNAs and 12 cDNAs without the ORF showing high similarity to the mitochondrial DNA of Trichinella spiralis. \ Conclusion \ Some cDNAs encoding antigenic protein of newborn larvae of Trichinella spiralis were obtained.