大豆异黄酮对骨代谢影响的组织学观察

The morphological study of the effect of genistein on bone metabolism

目的 探讨大豆异黄酮对牙槽骨、颌骨和胫骨骨代谢的作用 ,为临床上防治骨代谢性疾病提供实验依据。方法 切除成年雌性大鼠双侧卵巢造成绝经后骨质疏松的动物模型 ,并对大鼠进行四环素荧光双标记。光镜观察大鼠牙槽骨、颌骨的组织学改变 ;荧光显微镜下动态观察大鼠胫骨骨代谢的情况 ,并计算四环素双标记线间的平均距离 (distanceofdoublelabel,DDL)和矿化沉积速率 (mineralappositionalrate ,MiAR)。 结果 大豆异黄酮治疗后 ,牙槽嵴边缘和颌骨骨小梁附着较多的成骨细胞 ,骨质较致密 ,未见活跃的破骨细胞性骨吸收现象。荧光显微镜下 ,大豆异黄酮治疗组双标线减少、间距变窄 ,DDL和MiAR均有显著下降。结论 大豆异黄酮可以抑制破骨细胞性骨吸收 ,并对骨形成有一定的刺激作用 ,从而最终抑制了雌激素下降造成的骨转换率的增高 。

Objective To investigate the effect of genistein on the bone metabolism of alveolar bone,mandible and tibia in ovariectomized rats.Methods Forty 12 wk-old female SD rats were randomly assigned to four groups:①sham operated (Sham group);②ovariectomized (group OVX);③ovariectomized and treated with estradiol(group E);④ovariectomized and received genistein(group G).After 12 weeks,alveolar bone,mandible and tibia were obtained and sectioned.Before sacrificed,the mice were injected with 35mg/kg tetracycline.The sections were observed by light and fluorescent microscope (LM&FM).The indexes of distance of double label (DDL)and mineral appositional rate (MiAR)were calculated under FM. Results Histologically,there were more osteoblasts attached in the alveolar and mandible in group G and no osteoclastic bone resorption were observed after treated with genistein.Under FM,the amount of the double tetracycline line were decreased and the distance of double label were narrowed in group G.The indexes of DDL and MiAR were also decreased after treated with genistein.Conclusion Genistein can prevent osteoclastic bone resorption and stimulate bone formation in vivo histologically.It can be applied clinically in the prevention and treatment of mandible osteoporosis.