E2F-1和p21^(WAF1/CIP1)在良恶性多形性腺瘤中的定量表达和意义

Quantitive expression and significance of E2F-1 and p21^(WAF1/CIP1) in pleomorphic adenoma and malignant pleomorphic adenoma

目的 研究E2F - 1和 p2 1WAF1/CIP1在良、恶性多形性腺瘤中的表达和意义。方法 实验分为正常组、多形性腺瘤组 (PA)和恶性多形性腺瘤组 (MPA)。利用免疫组化和原位杂交的方法对三组分别进行E2F - 1和p2 1WAF1/CIP1的染色 ,并进行灰度值测量和统计学分析。结果 E2F - 1主要表达在肿瘤性上皮组织 ,软骨样细胞和粘液样组织中也有少量表达。在MPA组中的表达显著高于PA组 ,二者与正常组之间也有显著性差异。P2 1WAF1/CIP1主要在腺管状结构、上皮条索和团块鳞状化生结构的细胞浆中表达 ,在PA和MPA组中的表达均较弱 ,但MPA组比PA组更弱。结论 E2F - 1和p2

Objective To study the expression and significance of E2F-1 and p21 WAF1/CIP1 in the pathogenesis of pleomorphic adenoma and malignant pleomorphic adenoma.Methods Samples were divided into normal group, pleomorphic adenoma (PA) and malignant pleomorphic adenoma group (MPA). E2F-1 protein and p21 WAF1/CIP1 mRNA were stained by immunohistochemistry and in situ hybridization separately. The grey values were measured by image analysis system and analyzed by statistic method.Results Positive signals of E2F-1 were mainly found in neoplastic epithelium. E2F-1 was also expressed in chondroid and myxiod areas. Expression of E2F-1 was much higher in group MPA than in group PA. There was also a significant difference between normal group and group PA, MPA (P< 0.05). Staining of p 21WAF1/CIP1 appeared in ductlike spaces, strands epithelium and squamous epithelial cells. Staining of p21 WAF1/CIP1 was weak in group PA and MPA. However, Expression of p21WAF1/CIP1 was much weaker in group MPA than in PA group(P< 0.05). Conclusion E2F-1 and p21 WAF1/CIP1 can be useful indicators to evaluate the degree of malignance of pleomorphic adenoma.