VEGF蛋白检测对预测鼻咽癌远处转移风险的价值

Clinical Value of Vascular Endothelial Growth Factor Detection in Forecasting Distant Metastasis Risk of Nasopharyngeal Carcinoma

背景与目的:血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)是最重要的血管生长促进因子,并与多种肿瘤的恶性度及转移关系密切。本研究旨在通过同时检测鼻咽癌组织及血浆中的VEGF蛋白,评价其对预测鼻咽癌远处转移风险的价值。方法:同时收集59例鼻咽癌患者治疗前鼻咽癌组织及血清标本,用免疫组化LSAB法检测鼻咽癌组织中的VEGF蛋白,用ELISA法检测血清中的VEGF蛋白;并随访了解患者的远处转移情况,分析VEGF蛋白水平与患者远处转移的关系。结果:鼻咽癌组织及血清中的VEGF蛋白水平与远处转移均成正相关(均P<0.05)。鼻咽癌组织中VEGF蛋白表达阴性(-)、弱阳性(+)、阳性(++)和强阳性(+++)组患者的3年无转移生存率分别为100%、95.24%、65.19%、58.93%,各组之间有显著性差异(P=0.0193)。血清VEGF蛋白水平<466.78ng/L和≥466.78ng/L组的3年无转移生存率分别为82.57%,59.11%,两组比较有显著性差异(P=0.0211)。多因素分析发现鼻咽癌组织及血清VEGF蛋白水平对鼻咽癌远处转移都是独立的影响因素。结论:鼻咽癌组织及血清中VEGF蛋白检测均可用于判断鼻咽癌患者远处转移风险,二者联合检测可提高预测患者出现远处转移的能力。

BACKGROUND &OBJECTIVE:Vascular endothelial growth factor (VEGF) cl osely relates with the malignancy and metastasis of various kinds of cancers. Th is study was designed to evaluate the value of VEGF detection in forecasting dis tant metastasis risk of nasopharyngeal carcinoma(NPC) patients. METHODS: Tumor b iopsies,and serum specimens were collected before treatment from 59 pathological ly diagnosed NPC patients, hospitalized in our hospital from Mar. 1999 to Feb. 2 000. Immunohistochemistry LSAB method was used to detect VEGF in NPC tissues. VE GF in NPC serum was detected by quantitative enzyme-linked immunosorbent assay (ELISA) method. Through follow-up study, the relationship between VEGF and dist ant metastasis of NPC patients was observed. RESULTS: The expression of VEGF in NPC tissue,and serum positively related with the distant metastasis (P< 0.05). Non-distant metastasis survival rates of different tissue VEGF groups were 100 %(-), 95.24%(+), 65.19%(++),and 58.93%(+++),significant diffe rence existed between every 2 groups (P=0.019 3). Non-distant metastasis surviv al rates of different serum VEGF groups were 82.57%(< 466.78 ng/L) ,and 59.11% (≥466.78 ng/L)(P=0.021 1). Multi-prognostic factors analysis showed that VEGF expression in NPC tissue and serum had significant effects on distant metastasis of NPC. CONCLUSIONS: The detection of VEGF either in NPC tissue or in serum cou ld be regarded as a valuable indicator for predicting distant metastasis risk of NPC patients. Combined detection of them could increase the chances of forcasti ng distant metastasis in NPC patients.