人脑星形细胞瘤组织中NF-κB的DNA结合活性

DNA Binding Activity of Nuclear Factor-Kappa B in Human Astrocytoma

背景与目的:人类肿瘤细胞的转录因子可在转录水平调控许多恶性相关基因的表达,与肿瘤细胞的低分化和高转移有关,并抑制肿瘤细胞凋亡。本研究旨在观察人脑星形细胞瘤组织中核因子-κB(nuclearfactor-kappaB,NF-κB)的DNA结合活性,探讨其在人脑星形细胞瘤发生和发展中的意义。方法:采用电泳迁移率(electrophoreticmobilityshiftassay,EMSA)同位素放射自显影方法分别检测12例正常脑组织及37例人脑星形细胞瘤组织中NF-κB的DNA结合活性。结果:NF-κB的DNA结合活性在人脑星形细胞瘤组织光密度值为134.2±24.1,高于正常脑组织的97.5±1.9(P<0.05),其中多形胶质母细胞瘤(Ⅳ级)NF-κB的DNA结合活性最高,为106.8±7.4,依次为间变性星形细胞瘤(Ⅲ级)123.2±10.1,星形母细胞瘤(Ⅱ级)139.3±16.8,星形细胞瘤(Ⅰ级)160.2±18.6(P<0.05)。结论:NF-κB的DNA结合活性与人脑星形细胞瘤分级有关,并随恶性程度增加而增强。

BACKGROUND &OBJECTIVE: Transcription factors of human cancer cells can regulate the expression of many malignancy-related gene products, relate t o poor differentiation, and high metastasis of cancer cells, and also inhibit ap optosis of cancer cells. This study was designed to investigate the nuclear fact or-kappa B (NF-闎) DNA binding activity in human astrocytoma tissue, and its significance with human astrocytoma development. METHODS: The NF-闎 DNA bindin g activities of 12 cases of normal brain tissue, and 37 cases of human astrocyto ma tissue were detected by electrophoretic mobility shift assay (EMSA),and photo densitometry. RESULTS: The absorbency of NF-闎 DNA binding activity in human astrocytoma tissue was 134 2±24 1,higher than that in normal brain tissue (97 5±1 9)(P< 0 05). The NF-闎 DNA binding activity in multiform glioblastoma tissue was highest (106 8±7 4), that in anaplasia Astrocytoma was 123 2±10 1, in astroblastoma was 139 3±16 8, and in astrocytoma was 160 2±18 6 ( P< 0 05). CONCLUSION:The NF-闎 DNA binding activity correlates with malignan cy degree of human astrocytoma.