摘要
本论文通过酶法改性大豆蛋白,获得了在pH4.0条件下溶解良好的大豆蛋白.实验结果表明在1～3h的反应时间内,蛋白质经酶解达到了最大的的酸溶解性.最佳酶解工艺条件为:大豆分离蛋白浓度5%,酶用量5%(以反应物为100%计),pH8.0,55℃,反应时间3.5h.在此条件下,大豆蛋白的水解值达到了10.35%.在实验中进一步通过采用SDS-PAGE电泳方法测定大豆蛋白酶解情况及产物分子量范围.
The dissolubility of the soy protein in acid condition is becoming an important problem to be solved. The best time for the enzyme hydrolysis and the best combination of conditions are studied. Result indicated that the best combination of parameters for Alcalase is 5%substrate,5%enzyme,pH8.0,55℃ and 3.5h. The molecule weight of the soy protein is estimated by SDS-PAGE.
