摘要
本实验在高压环境(酸性条件)对海蛰进行降解,然后利用酶法对海蛰进行水解;并对各酶的酶解效果行了研究,同时摸索出了合适的加工工艺,成功研制出口服液。正交试验结果表明,采用碱性酶水解的最适条件为:温度,50 ,pH9.0,时间3.5h,加酶量2.0%,原料:水=1:2,蛋白质水解率达到34.1%。
In this experiment, the Rhopilema esculenta was hydrolyzed in high pressure with acidity, hydrolysate solution was go on to be hydrolyzed with enzyme ; The kind of enzyme, effect of hydrolysis and the optimal technique were studied; Through the orthogonal experiment the optimal condition for the hydrolysis with enzyme was determined to be 50 ,pH9.0,taking time 3.5h,enzyme concentration 2.0%, the proportion between Rhopilema esculenta kishinouye and water 1:2(on weight basis). The degree of hydrolysis was 34.1%.
