胰蛋白酶抑制剂抗虫基因转化烟草的研究

Studies on TI Gene Transformation to the Tobacco Plants

实验用携带质粒pAM194/TI的根癌农杆菌LBA4404转化烟草,通过筛选,共获得35株卡那霉素抗性苗.GUS组织染色、PCR检测及抗虫试验表明胰蛋白酶抑制剂抗虫基因已经成功地整合进再生植株染色体基因组中,有些已正确表达.

A sweet potato trypsin inhibitor(TI) gene was introduced into tobacco plants(Nicotiana tabaccu)by Agrobacterium tumefaciens-mediated leaf disc transformation.By plant tissue culturing and differentiation induction,35 kanamycin-resistant tobacco plant individuals were selected.GUS expression test and PCR amplification analysis showed that GUS reported gene and TI gene were successfully integrated into genome of all 35 independent kanamycin-resistant tobacco plant individuals and were normally expressed.In insecticidal bioassays of TI-transgenic tobacco plants,larval,the tobacco cutworm,was severely retarded while the control plants were vulnerable to it.This observation implied that Agrobacterium tumefaciens-mediated transformation and expression of sweet potato trypsin inhibitor can provide an efficient method for tobacco crop protection and production.