正交设计在毛白杨组织培养中的应用

Application of Orthogonal Design in Tissue Culture of Populus tomentosa Carr.

用正交设计法考察了4种激素对毛白杨叶片诱导愈伤组织的影响.结果显示,2,4 D,NAA作用显著,IAA,6 BA作用不显著.它们作用大小依次为:2,4 D>NAA>6 BA>IAA.诱导毛白杨愈伤组织产生的最佳激素配比为:MS+2,4 D1mg/L+6 BA1.5mg/L+IAA0.5mg/L.

Effects of 2,4-D, 6-BA, NAA and IAA on callus inducement of Populus tomentosa Carr. was studied by L_9(3~4) orthogonal design. The experimental results showed that the order of their efficiencies was 2,4-D>NAA>6-BA>IAA. The best medium for callus inducement was MS with 2,4-D(1 mg/L,) 6-BA1.5 mg/L and NAA0.5 mg/L.