正交设计研究杭白菊总提取物及总黄酮提取工艺

Study on extraction technology for extract and flavonoids in Chrysanthemum morifolium by orthogonal design

目的 :确定杭白菊总提物、总黄酮的提取工艺条件。方法 :采用正交试验法 ,以浸膏得率和总黄酮含量为考察指标 ,对杭白菊的超声提取条件进行优选。结果 :所考察的因素中 ,杭白菊的提取工艺影响程度为 :提取次数 >溶剂浓度 >超声时间 >溶剂量。结论 :超声提取的最佳条件为 :A2 B3 C3 D3 。与常规的提取方法相比 ,具有提取时间短、简单、收率稳定。

Objective: To ascertain extraction technology condition for extract and flavonoids from Chrysanthemum morifolium. Method: The optimizing ultrasonic extraction condition on the basis of extractive yield and flavonoids were determined by orthogonal design. Result: The order of factors which affected the flavonoid extraction was extraction times> ethanol concentration> ultrasonic time >solvent quantity. Conclusion: The optimum ultrasonic extractions are A_2B_3C_3D_3.Compared with traditional extraction,ultraction method is timesaving, simple to operate, stable and need not be heated.