龙胆泻肝汤对大鼠肾毒性的实验研究

Study rat nephrotoxicity of Longdan Xiegan Tang

目的 :观察和研究龙胆泻肝汤对大鼠肾毒性的影响 ,为该药临床应用的安全性提供实验数据。方法 :取大鼠 30只 ,随机分为给药组和对照组。各组相应处理后观察大鼠第 1,2 ,3,4周末 2 4h尿蛋白定量 (MTP)、MTP与尿肌酐 (Cr)比值、血尿素氮 (BUN)、血肌酐 (Cr)及肾皮质丙二醛 (MDA)含量 ,肾皮质超氧化物岐化酶 (SOD)活性等指标。通过光镜、电镜观察大鼠肾小球 ,肾小管结构的改变。结果 :灌服龙胆泻肝汤的大鼠 ,在给药后 1～ 3周内尿蛋白明显上升 ,MTP ,MTP/尿Cr与对照组比P <0 .0 1;血BUN与对照组相比 ,P <0 .0 5 ;血Cr、肾皮质MDA、肾皮质SOD与对照组相比 ,P >0 .0 5。光镜检验显示 :2组各有一处局灶性间质性肾炎 ,其余各肾脏组织学结构均未见异常。电镜检查显示 :灌服龙胆泻肝汤样本均见基底膜有节段增厚外凸 ,但未见沉积物。部分样本见足突小部分融合 ,肾小管上皮未见异常 ,少数小管腔内可见脱落细胞。结论

Objective: To investigate the nephrotoxocity of Longdan Xiegan Tang in rats, and to test its safety. Method: Sprague-Dauley rats were given Longdan Xiegan Tang 4.5 mL·(100 mg)^(-1) Bid for thirty days , and the control group was given NS. MTP, BUN, Cr, MDA, MTP/Ucr and SOD were measured on 1st, 2nd, 3rd, 4th week. The kidney tissues were viewed with light microscopy and electron microscope. Result: MTP and MTP/Ucr were obviously higher than controls ( P<0.01), and the other index had no difference (P>05). No remarkable structural change could been seen with light microscopy, but with electron microscope we could find that the basal membranes were thickened and some of foot process were infused. Conclusion: Longdan Xiegan Tang will result in injury of kidney function.