2型糖尿病患者慢性牙周炎的细菌学特点

Bacteriological Features of Patients with Chronic Periodontitis and Type-2 DM

为研究 2型糖尿病患者慢性牙周炎的细菌学特点及其与血糖、糖化血红蛋白的关系 ,应用细菌学厌氧培养、PCR检测技术 ,对 42例 2型糖尿病慢性牙周炎患者和 2 1例健康人的龈下菌斑进行专性及兼性厌氧培养并计数。发现 :2型糖尿病患者慢性牙周炎的龈下细菌中牙周优势菌为产黑菌、二氧化碳噬纤维菌、核梭杆菌、中间普氏菌 ;产黑菌、二氧化碳噬纤维菌数量随患者空腹血糖、糖化血红蛋白的升高而增加。提示 :2型糖尿病患者慢性牙周炎的龈下菌群以厌氧菌为主 。

Bacteriological changes of subgingival flora of patient with chronic periodontitis and type-2 DM(diabetes mellitus) were researched by germ cultivation and PCR method in the thesis. Samples were taken from 42 patients with chronic periodontitis and type-2 DM, and 21 normal people. An isolated cultivation of subgingival plaque samples was performed by a anaerobe non-selected media, the Actinomyces, Fnusbacterium nucleatum, Eikenella corrodens, A. actinomycetemcomitans, capnocytophaga and Streptococcus spp were performed by selective media. The P.intermedia(P.i) of the black colony in the non-selective media was detected by PCR. The quantity of germs was recorded.It was discovered that the major germ of subgingival flora of type-2 DM patients with chronic periodontitis was anaerobe, the superiority germ was black germs, P.i, Fnusbacterium nucleatum and capnocytophaga. Statistical analysis of correlation between FPG、GHb and amount of black germs, capnocytophaga was made. It shows that chronic periodontitis is correlated to type-2 DM.