摘要
研究了南瓜多糖提取液的分离与纯化。试验结果表明 ,提取液用TCA法脱蛋白 ,去除率达 94.3 3 % ,采用SephacrylS 40 0HR凝胶柱层析逐步分析纯化 ,分离得到 2种多糖PP Ⅰ和PP Ⅱ。利用醋酸纤维膜电泳法对PP Ⅰ和PP Ⅱ进行纯度检测 ,表明 2种组分都达到了均一的纯度。
The separation and purification of the polysaccharide from pumpkin was studied in this paper By using TCA method, 94.33% of protein was removed from the extracted juice. Further purification by Sephacryl S-400 HR gel-column produced two types of polysaccharide: PP-I and PP-II. The purity of PP-I and PP-II were examined by acetate fiber film electrophoresis method. The results show that the purity of two compositions are both homogeneous.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2004年第8期130-133,共4页
Food and Fermentation Industries
