细胞凋亡及sFas、sFasL在人嗜T-淋巴细胞病毒Ⅰ型相关性脊髓病/热带痉挛性瘫痪中的表达及其意义

The Apoptosis and the Expression of sFas、sFasL in Patients with Human T-lymphotropic Virus Type I-associated Myelopathy/Tropical Spastic Paraparesis

目的 :研究人嗜T淋巴细胞病毒Ⅰ型相关性脊髓病 /热带痉挛性瘫痪 (HAM/TSP)外周血淋巴细胞凋亡情况 ,以及患者血浆及脑脊液 (CSF)中sFas、sFasL的表达水平在HAM/TSP发生、发展中的作用。方法 :用单细胞电泳检测HAM /TSP患者外周血淋巴细胞的凋亡情况 ,ELISA方法检测HAM/TSP患者血浆及CSF中存在的sFas、sFasL。结果 :HAM/TSP患者外周血淋巴细胞凋亡率明显增高 ,与正常对照组比较有统计学意义 (P <0 0 5 ) ;患者血浆sFas、sFasL表达水平与其他神经系统感染性疾病 (OIND)及正常对照组相比显著增高 (P <0 0 5 ) ;血浆sFasL表达水平高于OIND及正常对照组 (P >0 0 5 ) ;CSF、sFas、sFasL表达水平均高于OIND组 (P <0 0 5 )。结论 :淋巴细胞凋亡及凋亡相关因子sFas、sFasL可能在HAM/TSP的发生。

Objective:To investigate the apoptosis of peripheral blood lymphocyte cells in patients with human T-lymphotropic virus type I-associated myelopathy/tropical spastic paraparesis(HAM/TSP)and the roles of sFas,sFasL protein in occurrence and progression of HAM/TSP.Methods:The single cell gel electrophoresis was used to detect the apoptosis of lymphocyte cells in patients with HAM/TSP.The presence of sFas and sFasL in the sera and CSF of patients with HAM/TSP was evaluated by an enzyme-linked immunosorbent assay.Results:The apoptotic ratio of lymphocyte cells in patients with HAM/TSP significantly increased,compared with con trol(P<0.05).Significantly increased serum levels of sFas were found in patients with HAM/TSP than those with other inflammatory neurological disorders(OIND) and control(P<0.05).Serum levels of sFasL from patients with HAM/TSP showed increasing trend compared with control or patients with OIND(P>0.05).Levels of sFas and sFasL in CSF from patients with HAM/TSP were both significantly increased than those from ones with OIND(P<0.05).Conclusion:Apoptosis of lymphocyte cells and the levels of sFas and sFasL can play an important role in the development of HAM/TSP.