创伤性休克大鼠血浆内皮素与一氧化氮的动态变化及山莨菪碱的治疗作用的实验研究

Study on dynamic changes of plasma NO、ET and therapeutic effect of anisodamin on traumatic shock in rats

目的观察创伤性休克过程中血浆一氧化氮(nitricoxide,NO)、内皮素(endothelin,ET)与组织氧分压的动态变化,探讨山莨菪碱在创伤性休克过程中的治疗作用。方法采用下肢创伤法建立创伤性休克大鼠模型,随机分为休克组与处理组,观察创伤前后血NO、ET及骨骼肌、肝脏、小肠的组织氧分压的动态变化,监测血液动力学变化并记录存活时间。结果创伤休克后血NO、ET浓度显著高于伤前水平,休克后组织氧分压较创伤前显著降低;处理组复苏后5、12h血浆ET浓度显著低于休克组,复苏后各时间点血NO/ET值高于休克组,复苏后各时间点肝脏、小肠氧分压显著高于休克组,处理组12、24h存活率显著高于休克组(P<005)。结论NO、ET及NO/ET的失衡在创伤性休克过程中有重要的意义,应用654-2可减少ET的分泌与释放,使NO/ET值升高,改善肝脏及小肠的氧分压,从而改善创伤性休克大鼠的预后。

Objective To observe the dynamic changes of nitric oxide(NO),endothelin (ET) and oxygen partial pressure in tissues under traumatic shock and to explore the therapeutic effect of anisodamin.Methods Animal models of traumatic shock in Sprague-Daulay rats were made and randomly devided into shock group and treated group.Dynamic changes of plasma ET、 NO and oxygen partial pressure in tissues of skeletal muscles,liver and small intestine were detected at pretrauma and postshock.Meanwhile,hemodynamic data of the rats and their survival time was monitored and recorded.Results The levels of plasma NO and ET increased while tissues oxygen partial pressure decreased significantly after traumatic shock. After resuscitation, plasma ET of treated group at 5,12 h were significant lower than that of shock group as well as its NO/ET after resuscitation were higher than that of shock group.Oxygen partial pressure in liver and small intestine of treated group after resuscitation were significantly higher than that of shock group and their survive rates in 12,24 h were higher than that of shock group (P<0 05). Conclusion There were important significance of the disorder of NO/ET during traumatic shock.Treatment of anisodamin could decrease plasma ET,raise NO/ET levels,improve oxygen partial pressure of internal organs and increase the survival rate of models significantly. [