4种肿瘤-睾丸抗原在肝细胞癌中的表达

Expression of cancer-testis antigen in hepatocellular carcinoma

目的 观察肿瘤睾丸(CT)抗原MAGE1、SSX-1、CTp11及HCA587基因mRNA在肝细胞癌(HCC)中的表达并探讨其对HCC免疫治疗的意义。方法 用逆转录-聚合酶链反应(RT-PCR)的方法对HCC患者癌组织和对照组织中的目的基因mRNA进行检测。结果在检测的105例HCC组织中,MAGE-1、SSX-1、CTp11、HCA 587基因mRNA阳性率分别是75.2％、72.4％、62.9％和56.2％:至少表达1种CT抗原者达93.3％;对照组仅有3例癌旁组织SSX-1基因mRNA表达为弱阳性。结论MAGE-1、SSX-1、CTp11及HCA 587基因mRNA在HCC中呈高比例、高特异表达,是HCC免疫治疗的理想靶位;多个CT抗原同时在HCC中表达;为HCC多价瘤苗的研制提供了实验依据。

Objective To investigate the expression of cancer-testis (CT) antigen MAGE-1, SSX-l,CTpll and HCA587 genes in hepatocellular carcinoma (HCC) and the possibility of applying these antigens as targets for specific immunotherapy for HCC. Methods The expression of MAGE-1, SSX-1, CTp11 and HCA587 mRNA was detected by using reverse transcription polymerase chain reaction (RT-PCR) in HCC tissues and the corresponding adjacent non-HCC tissues from 105 HCC patients, 40 samples of cirrhosis and normal liver tissues. Five samples selected randomly from each gene with positive PCR results were sequenced.Results Ninety-eight of the 105 HCC patients (93.3% ) were found to be positive for MAGE-1 (75. 2%, 79/105), SSX-1 (72. 4%, 76/105), CTpll (62. 9%, 66/105) or HCA587 (56.2%, 59/105) transcripts in HCC samples respectively. At least 2, 3 and 4 of these genes were positive in 72.4% (76/105), 48.6% (51/105) and 37.1% (39/105) of HCC tissues, respectively. Conversely, only SSX-1 could be detectable in 2.9% (3/105) of the corresponding adjacent non-HCC tissues in which no metastatic lesions were found. In addition, none of 40 samples of cirrhosis and normal liver tissue expressed each CT antigen gene mRNA. Conclusion CT antigens (MAGE1, SSX-1, CTp11 and HCA587) are expressed with high percentage and specificity in HCC and the promising targets for antigen-specific immunotherapy of HCC;High frequent co-expression of multiple members of CT antigens in HCC provides possibility of polyvalent vaccinations for HCC.