三七皂甙单体2A-1-1对人血小板聚集和钙内流的作用

Effects of 2A-1-1 on the aggregation and Ca^(2+) influx of platelets

目的 观察三七皂甙单体 2A 1 1对人血小板聚集和钙内流的影响 ,并探讨其对受体操纵性钙通道的作用。方法 比浊法测定血小板聚集 ;Fura 2 /Am荧光探针双波长测定细胞胞浆游离钙浓度 ,观察 2A 1 1、硝苯地平、SK&F96 36 5对二磷酸腺苷 (ADP)、环匹阿尼酸 (CPA)介导的人血小板钙内流的变化。结果 硝苯地平 (2 0 μmol/L)不能抑制ADP诱导的血小板聚集 ,不能抑制ADP或CPA介导的血小板钙内流 ;SK&F96 36 5 (2 0 μmol/L)可以抑制ADP诱导的血小板聚集 ,抑制率为5 9.83% ;SK&F96 36 5 (15 μmol/L)可以抑制CPA和ADP介导的钙内流 ;2A 1 1(5 ,10 ,2 0 μmol/L)可抑制ADP诱导的血小板聚集 ,抑制率分别为 4 7.0 6 % ,5 3.4 7% ,71.5 2 % ;2A 1 1(10 ,2 0 μmol/L)可抑制CPA和ADP介导的钙内流。结论 三七皂甙单体 2A 1 1能抑制人血小板聚集 ,抑制血小板受体操纵性钙通道 ,从而抑制钙内流 ,有抗血小板作用。

Objective To explore the effects of 2A-1-1 (purified component from Panax notoginsengs s aponins) on the aggregation of and Ca 2+ influx into human platelets. Methods The aggregation of platelets was tested by nephelometry, Fura- 2 fluorescent technique was used for detecting cell \i. The effects of 2A-1-1, nifedipine and SK&F96365 on Ca 2+ influx into human platelets induced by ADP or CPA were observed seperately. Results Nifedipine (<20 μmol/L) could not inhibit platelet aggregation induced by ADP or the Ca 2+ influx induced by ADP or CPA. SK&F96365 at 20 μmol/L could inhibit the m aximal aggregation of platelets induced by ADP with a inhibitory rate of 59.8 3%, at 15 μmol/L could inhibit the Ca 2+ influx induced by CPA or ADP. 2A-1-1(5, 10 and 20 μmol/L)could inhibit the maximal aggregation of platel ets induced by ADP with the inhibitory rates of 47.06%, 53.47% and 71.52%, respectively. 2A-1-1 at 10 and 20 μmol/L could inhibit the Ca 2+ influ x induced by CPA or ADP. Conclusions 2A-1-1 can inhibit platelets aggregation, block the ROC (Receptor-dependent Ca 2+ channels) and inhib it Ca 2+ influx of human platelets.