血管性痴呆发生与组织型纤溶酶原激活物活性的相关性

Correlation between the occurrence of vascular dementia and the activity of tissue type plasminogen activator

目的:研究组织型纤溶酶原激活物(tissue-typeplasminogenactivator,TPA)活性与血管性痴呆(vasculardementia,VD)的相关性,探讨其发生机制。方法:将SD大鼠随机分为手术组和假手术组,用结扎大鼠双侧颈总动脉建立VD模型,水迷宫测试,取脑匀浆用酶联免疫吸附法(ELISA法)测试TPA活性。结果:结扎后存活时间小于24h大鼠的TPA活性比结扎后存活时间8～31d大鼠的TPA活性高,结扎后存活时间小于24h的手术组TPA活性(5.535±1.119)比假手术组(5.348±0.848)高,差异无显著性意义(t=-0.396,P>0.05);结扎后存活时间8～31d的手术组TPA活性比假手术组低,差异有显著性意义(F=14.120,P<0.05)。结论:TPA活性的下降在早期可能参与了血管性痴呆的发生。

AIM:To study the relation of the activity of tissue type plasminogen activator(TPA) with vascular dementia(VD),and explore its pathogenesis. METHODS: SD rats were randomly divided into operation and sham operated groups, and VD models were established by ligation of bilateral common carotid arteries, then water maze test was conducted. The brain homogenate was taken to determine the TPA activity by using the enzyme linked immunoadsordent assay (ELISA). RESULTS: The TPA activity was higher in the rats which survived less than 24 hours after ligation(group A) than those which survived 8 to 31 days (group B).The TPA activity in group A(5.535±1.119) was higher than that in the sham operated group(5.348±0.848), and the difference was not significant(t=-0.396, P >0.05); the TPA activity in group B was significantly lower than that in the sham operated group(F=14.120,P< 0.05). CONCLUSION: The decrease of TPA activity may take part in the occurrence of VD in the early stage.