微波辐照对小鼠脑乳酸脱氢酶和一氧化氮合酶活性及丙二醛含量的影响(英文)

Effects of microwave radiation on the activity of lactate dehydrogenase and nitric oxide synthase and the content of malondialdehyde in the brain of mice

背景:研究手机微波是否对人体健康有潜在危害已成为热点问题。目的:观察不同频率和不同发射功率的微波照射对小鼠脑丙二醛含量、乳酸脱氢酶(lactatedehydrogenase,LDH)和一氧化氮合酶(nitricoxidesynthase,NOS)活性的影响。设计:随机对照的实验研究。地点和对象:实验地点:首都医科大学动物科学部。按随机数字表法将50只无特定病原体(SPF)C57BL/6N小鼠分为正常对照组和45MHz组(新式手机组):照射功率20mW/cm2,照射时间12h/d;450MHz组(老式手机组):照射功率100mW/cm2,照射时间6h/d;870MHz组(无绳电话组):照射功率100mW/cm2,照射时间6h/d;2450MHz(强微波组):照射功率730mW/cm2,照射时间2s/次,3次/d。每组10只。干预:采用不同辐照强度的微波照射3个月后颈椎脱臼处死,用硫代巴比妥酸(TBA)比色法、二硝基苯肼显色法(DNPH比色法)和吩嗪二甲脂比色法(PMS显色法)分别检测小鼠脑组织中脂质过氧化产物-丙二醛含量、LDH和NOS活性。主要观察指标:①强微波辐照时小鼠脑中丙二醛含量、LDH和NOS的活性。②弱微波辐照时小鼠脑中丙二醛含量和LDH的活性。结果:2450MHz强微波辐射后,丙二醛含量犤(29.571±3.888)μmol/g犦升高,与对照组犤(21.660±2.729)μmol/g犦比较,差异有统计学意义(Z=1.789,P<0.01),LDH和NOS活性犤(17.

BACKGROUND:It has become a hot spot problem to explore if the microwave of mobile phone does latent harm to the body health. OBJECTIVE:To discuss the effect of microwave radiation at different frequencies and different levels of power on the content of malondialdehyde and the activity of lactate dehydrogenase(LDH) and nitric oxide synthase(NOS) in the brain of mice. DESIGN:A randomized controlled experimental research. SETTING and MATERIALS:Experimental setting: Animal Science Department of Capital University of Medical Sciences.According to random number table,50 specific pathogen free(SPF) C57BL/6N mice were divided into the following groups:normal controlled group and 45 MHz group(new style mobile phone group):irradiation power 20 mW/cm2,irradiation time 12 hours/day;450 MHz group(old style mobile phone group):irradiation power 100 mW/cm2 ,irradiation time 6 hours/day;870 MHz group(wireless telephone group):irradiation power 100 mW/cm2,irradiation time 6 hours/day; 2 450 MHz group(strong microwave group):irradiation power 730 mW/cm2,irradiation time 2 s each time,3 times a day.There were 10 mice in every group. INTERVENTIONS:The mice were put to death by dislocation of the cervical vertebra after having been irradiated for 3 months by microwave of different intensities of radiation.The content of Lipid peroxidation product malondialdehyde and activity of LDH and NOS in the brain tissue of mice were detected by thiobarbital acid(TBA)chromatometry,dinitrophenylhydrazine coloration(DNPH chromatometry),and azophenylene dimethyl ester chromatometry(PMS coloration) MAIN OUTCOME MEASURES:①The content of malondialdehyde and the activity of LDH and NOS in the brain of mice irradiated by strong microwave.②The content of malondialdehyde and the activity of LDH and NOS in the brain of mice irradiated by weak microwave. RESULTS:After irradiation of strong microwave at 2 450 MHz,the content of malondialdehyde increased[(29.571±3.888) μmol/g]. The difference showed statistical significance(Z=1.789,P< 0.01),compared with the control group[(21.660±2.729) μmol/g].The activity of LDH and NOS was[(17.560±1.827) μkat/g and(7.168 ±1.650)nkat/g]respectively.There was no statistical significance(P >0.05),compared with the control group[(20.996±4.919) μkat/g and(6.885±1.684)nkat/g]. After 870,450 and 45 MHz weak microwave irradiation,there was no great change in the content of malondialdehyde and the activity of LDH.There was no statistical significance(P >0.05). CONCLUSION:Under the condition of strong irradiation(2 450 MHz),microwave produces overdose of free radicals in the brain tissue which take part in the brain injury.While under the condition of weak irradiation(870,450,45 MHz),microwave basically does no harm to the brain.So it is safe to use mobile phone.