补肾益气和活血祛瘀中药治疗血管性痴呆的实验研究(英文)

Experimental study of Chinese herbs for replenishing kidney,benefiting qi,activating blood circulation to remove stasis in treatment of vascular dementia

背景:血管性痴呆(vasculardementia,VD)是老年期痴呆中常见的一种。补肾益气法、活血祛瘀法是治疗VD的常用中医治疗方法。而对这两种方法的比较研究尚少。目的:比较补肾益气方、活血祛瘀方对血管性痴呆大鼠的治疗作用,并探讨中药治疗血管性痴呆大鼠的机制。设计:完全随机设计,对照实验研究。地点与材料:地点为浙江省中医院脑病研究室。材料为清洁级健康成年雄性SD大鼠75只,鼠龄18～20月,体质量(400±30)g。由浙江省中医学院实验动物中心提供并饲养。方法:采用双侧颈总动脉永久性结扎造成血管性痴呆大鼠模型,并观察药物对该大鼠模型的学习记忆能力,海马CA1区锥体细胞的形态及数量的影响。结果:①补肾益气组大鼠跳台测试成绩,造模前(2.24±0.17)s,造模用药后59d(2.73±0.34)s,造模用药后60d(2.35±0.28)s。活血祛瘀组大鼠跳台测试成绩,造模前(2.41±0.57)s,造模用药后59d(2.62±0.41)s,造模用药后60d(2.34±0.44)s。补肾益气方,活血祛瘀方可明显改善血管性痴呆大鼠学习记忆能力。②补肾益气方,活血祛瘀方有减轻缺血对海马CA1区锥体细胞损伤的作用。结论:①中药补肾益气方、活血祛瘀方为治疗血管性痴呆大鼠的有效方,但活血祛瘀方的治疗作用更好。

BACKGROUND:Vascular dementia(VD)is commonly seen in senile dementia.The methods of replenishing kidney and benefiting qi and activating blood circulation to remove stasis are the common therapies in treatment of VD with Chinese medicine.But there are fewer researches on the comparison of the two methods. OBJECTIVE:To compare the effects of the recipe for replenishing kidney and benefiting qi(Recipe No.1)and the recipe of activating blood circulation to remove stasis(Recipe No.2)and probe into the mechanism of Chinese herbs in treatment of VD in rats. DESIGN:Completely randomized,controlled trial design. SETTING and MATERIALS:The experiment was performed in Research Room of Encephalopathy of Zhejiang Hospital of Traditional Chinese Medicine.Seventy five normal SD male rats, in clean class, aged varied from 18 months to 20 months,weighing(400±30)g were employed, provided and bred by the Experimental Animal Center of Zhejiang Hospital of Traditional Chinese Medicine. INTERVENTIONS:Permanent ligature on bilateral common carotid arteries were done to made VD models in rats.It was to observe the effects of the medicine on learning and memory in rat model and on morphology and quantity of pyramidal cells in hippocampal CA1 area. RESULTS:①The diving scores in Recipe No.1 group:(2.24±0.17) s before model making,(2.73±0.34) s 59 days after medication in model making and(2.35±0.28) s 60 days after medication in model making.The diving scores in Recipe No.2 group:(2.41±0.57)s before model making,(2.62±0.41) s 56 days after medication in model making and(2.34 ±0.44) s 60 days after medication in model making.Recipe No.1 and No.2 remarkably improved learning and memory of VD rats.②Recipe No.1 and No.2 can reduce the ischemic injury of pyramidal cells in hippocampal CA1 area,and such action was more remarkable in Recipe No.2. CONCLUSION:①Both Recipe No.1 and No.2 are the effective formulas in treatment of VD in rats,and Recipe No.2 can achieve better results.②The mechanism of Recipe No.2 is probably related to reducing the ischemic injury of pyramidal cells in hippocampal CA1 area.