KM－1d突变株小鼠的模型建立及遗传分析

Establishment and Genetic Analysis ofKM-1d Mutant Stock

在饲养经剖腹产净化后的ＫＭ种群时，我们发现了一些后肢畸形的动物、通过挑选后已成为一个稳定遗传的突变株，由于此群体产生的后代１００％均为后肢畸形动物，因此可以认定为１ｄ／１ｄ纯合子动物。命名为ＫＭ—１ｄ小鼠。将ＫＭ－１ｄ纯合子动物与近交系ＤＢＡ／２小鼠杂交得到Ｆ１代，再经Ｆ１代互交或与双亲回交。通过对后代的形态学观察及遗传方式的分析，证明为常染色体隐性单基因遗传。另外，对１３８只ＫＭ－１ｄ畸形小鼠进行解剖观察还发现有４２％的动物在骨骼畸形的同时伴有左侧泌尿系统畸形。因此可以认定ＫＭ－１ｄ小鼠是一种患有骨－肾先天性畸形综合症的动物模型。

imb deformity was found in SPF KM stock,whichis due to the increaseof inbreeding coefficent after caeserean operation.The skeletal deformities ofKM-ld appear to be confined to the portions of limbs below the elbows andknees.The tibia and fibala and the ulne and radius are shorter than that ofnormalones,or replaced by single curved bones in soine homozygotes.Preaxialpolydactyly and syndactyly of hindfeet were also found in most KM-ld hom-ozygotes.Among 138 KM-ld mice,42% were combined with anomalies of lefturogenital system, including anephrogenesis,hydronephrosis and hydroureter inthe left side.Breeding analysis data are 21 :121 (X2 3.16) by intercrosses and14:27 ( X20.04 ) by backcrosses. Therefore,ld is autosome single gene recessiveinherited and KM-ld stock will be useful model in biomedical research.