用Southern印迹技术检测9种恶性淋巴瘤细胞系IgCλ基因的重排(英文)

Detection of Immunoglobulin Cλ Gene Rearrangementin Nine Kinds of Malignant Lymphoma Cell LinesUsing Southern Blotting Technique

成熟B细胞具有Ig重链和轻链的基因重排,而大多数非B细胞来源的造血细胞仍维持在胚系结构.这种Ig基因重排可作为一个敏感的特异指标,即使小于5%克隆性细胞群,也可证明其克隆性.淋巴瘤为恶性克隆起源的血液病,Ig基因的重排测定可以帮助其分型和预示早期复发.本文用Southern印迹技术分析人9 种恶性淋巴瘤细胞系,并于正常胚系DNA比较.这9种恶性淋巴瘤细胞系由美国国立癌症研究所提供,均为B细胞恶性淋巴瘤.实验结果表明,正常胚系DNA lgCλ基因表现为14Kb,8Kb,5Kb片断,SU-DHL-2细胞系表现为重排,比正常胚系细胞多出一个 4Kb片断,NU—DHL—1细胞系亦为重排,分别为13.5Kb,7Kb,4.9Kb和3Kb.Raji细胞系亦有3Kb重排,SB细胞系表现为13,5Kb,7Kb,4.7kb重排,Daudi细胞系亦是如此.8392细胞系缺失5Kb片断,仅SU-DHL-4,SU-DHL-5,PA-3细胞系维持胚系结构.每种细胞系个体的特异性重排是其恶性克隆起源的基因标志,这对于了解B淋巴细胞肿瘤生成,发展,演变的机制有重要意义.B 淋巴细胞起源的恶性肿瘤中,Cλ基因的重 排为20%～75%,而T淋巴细胞起源的恶性肿瘤中,Cλ基因的重排为0%.在髓系起源的细胞中亦无Cλ基因的重排,因此Cλ基因的重排可鉴别T,B细胞的起源和淋,粒细胞的起源.因为每种恶性克隆均有自己的Cλ基因的重排方式。

Nine kinds of malignant lymphoma cell lines and normal germline DNA using southern blotting technique were compared. Cλ gene which derived from normal germline DNA showed 14Kb, 8Kb and 5Kb fragments. Weanwhile, rearrangement emerged from SU-DHL-2, NU-DHL-1, Raji, SB, and Daudi cell lines. Partial deletion appeared in 8392 cell line. Only SU-DHL-4, SU-DHL-5, PA-3 cell lines had germline configuration. The individual immunoglobulin gene rearrangement in every line provided a gene marker which originated from malignant clone. The important significance of this study contributed to understanding the mechanism of growth, development and evolution in B-cell neoplasmas, differentiaiting myeloid and lymphoid cells and malignant origin of T, B cells, establishing gene typings, detecting drug sensitivity, and finding minimal residual disease.