刺玫制剂对氧应激的生物学防护效应研究

Study on the biological protective effect of the preparation of Rosa Davurica Pall on the oxidative stress

[目的] 探讨刺玫制剂对氧应激是否有生物学防护作用。[方法] 动物试验分为3个剂量组,进行对由CC14诱发的自由基介导的肝损伤的保护作用试验;自由基介导的辐射损伤保护效应试验;抗氧化动物试验和果蝇生存试验。[结果] 刺玫制剂0.8g/kg组的GOT和GPT含量与CC14模型组比较均显著降低;动物病理组织学检查积分与CC14模型组比较显著降低;存活时间与存活率与对照组相比,显著提高;MDA与对照组相比,显著降低;SOD活性与对照组相比,显著增高;1.08%剂量组雄性果蝇的半数死亡时间和平均寿命及雌性果蝇的平均寿命与对照组相比,均显著延长。[结论] 刺玫制剂分别对CC14和辐射诱发的活性氧介导的肝细胞损伤,动物死亡具有防护作用;可降低衰老大鼠的氧应激水平;

Objective] To explore whether the preparation of Rosa Davurica Pall (RDP) has biological protective effect on the oxidative stress. [Methods] Three different doses were used to test animals for protecting liver, anti-radiation,anti-oxidation and surrival time of drosophila. [Results] The value of GOT and GPT and the pathology tissue integral of the high dose group decreased obviously in the study group as compared with that of the control.The survival time and rate of the study group increased obviously compared with that of the control,while the SOD activity increased obviously. The subduple death time and average lifespan in the 1.08% dosage of the male drosophila extend obviously as compared with that of the control,so does the average lifetime of female drosophila. [Conclusion] The preparation of Rosa Davurica Pall(RDP) has protective effect toward the injuried liver cell from reactive oxygen spicices respectively with CCl 4 and radiation,protects the animals from death,reduces the oxidative stress level of the senescent rat,so as to consider that RDP extent the lifespan of the drosophila is possibly concerned with reducing the oxidative stress level.The preparation of RDP has biological protective effect of reducing the oxidative stress level.