几种抗生素对离体培养中植物形态发生的影响

Effects of Several Antibiotics on the Morphogenesis of Explants Cultured in vitro

抗生素羧苄青霉素(Carbenicillin),头孢霉素Ⅱ(Keflodin)和Claforan影响甘蓝型油菜(Brassica napus L.)游离小孢子培养胚胎发生,其浓度越高,影响越大.羧苄青霉素促进辣椒(Capsicum annuum L.)子叶外植体愈伤组织发生,同时,还影响油菜小孢子胚再生植株及辣椒子叶再生株的生根,产生粗短根甚或发育为瘤状物.

The morphogenesis of explants cultured in vitro was affected by three different antibiotics, i.e. Carbenicillin, Claforan, and Keflodin. All of them showed an unfavorable effect on microspore embryogenesis in isolated microspore culture of Brassica napus cv. Topaz. For Carbenicillin, within the range of 0-500mg/L, the higher the concentration, the less the embryos were produced. The best concentration of Carbenicillin seemed to be 50mg/L for inhibiting bacteria growth and achieving favourable production of embryos. Carbenicillin also had some stimulative effects on callus formation of pepper cotyledon explants, and on rooting of regenerated plants resulting in shorter and thicker roots or even roots with tumors.